Coding

Part:BBa_K1842000:Design

Designed by: Walter Thavarajah   Group: iGEM15_UC_San_Diego   (2015-08-17)


LuxABfrp


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1592
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 955
    Illegal AgeI site found at 1549
    Illegal AgeI site found at 2854
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was too long to be directly synthesized, so we had to synthesize it in fragments that were joined through Gibson Assembly.


Source

The luciferase used is a synthetic construct, modified from Photorhabdus Luminescens luciferase and engineered for enhanced activity and thermostability. The NADPH-flavin oxidoreductase used was isolated from Vibrio Harveyi.

References